Fast-moving cell processes can be seen in 3D for the first time
The new lattice sheet microscope shoots high-res pictures of biological processes so quickly that fast-moving cells can be seen in 3D for the first time. The hardware is even able to produce video cli...
Exploring the universe of the cell; The 2012 GE Healthcare Cell Imaging Competition images
An animated film of the beautiful cell images that competed in the 2012 Cell Imaging Competition; first shown in Times Square New York, 20th April 2013. GE H...
'Gentle' microscope captures tiny life in action
Microscopists have been able to peer deep into cells, thanks to fluorescent molecules that stick to cellular structures. But the powerful light sources—often lasers—required to activate the fluorescen...
Cell imaging - Microscopic cell imaging jumps to 3D movies; unobtainium not required
Looking at cells just got a whole lot more interesting than just looking at a flat, super magnified image. Two researchers from the École Polytechnique Fédérale de Lausanne (EPFL) have f...
Staining
Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological t...
Staining - Wikipedia
Live cell imaging
Live cell imaging is the study of living cells using time-lapse microscopy. It is used by scientists to obtain a better understanding of biological function through the study of cellular dynamics. Liv...
Live cell imaging - Wikipedia
Phase contrast microscopy
Phase contrast microscopy is an optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image. Phase shifts themselves are ...
Phase contrast microscopy - Wikipedia
Digital holographic microscopy
Digital holographic microscopy (DHM) is digital holography applied to microscopy. Digital holographic microscopy distinguishes itself from other microscopy methods by not recording the projected image...
Digital holographic microscopy - Wikipedia
Fluorescence microscope
A fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to study properties of organic or inorganic subst...
Fluorescence microscope - Wikipedia
Light sheet fluorescence microscopy
Light sheet fluorescence microscopy (LSFM) is a fluorescence microscopy technique with an intermediate optical resolution, but good optical sectioning capabilities and high speed. In contrast to epifl...
Light sheet fluorescence microscopy - Wikipedia
Lattice light-sheet microscopy
Lattice light-sheet microscopy is a modified version of light sheet fluorescence microscopy that increases image acquisition speed while decreasing damage to cells caused by phototoxicity. This is ac...
Fluo-4
Fluo-4 is used to measure calcium (Ca) concentrations inside living cells, and is often used for high-throughput screening of receptor ligands and calcium permeable ion channels.The green-fluorescent ...
Fluo-4 - Wikipedia
Fura-2
Fura-2, an aminopolycarboxylic acid, is a ratiometric fluorescent dye which binds to free intracellular calcium. It was the first widely used dye for calcium imaging, and remains very popular. Fura-2...
Fura-2 - Wikipedia
Multiphoton fluorescence microscope
A multiphoton fluorescence microscope (MFM) is a specialized optical microscope.
The MFM uses pulsed long-wavelength light to excite fluorophores within the specimen being observed. The fluoropho...
Second-harmonic imaging microscopy
Second-harmonic imaging microscopy (SHIM) is based on a nonlinear optical effect known as second-harmonic generation (SHG). SHIM has been established as a viable microscope imaging contrast mechanism...
Fluorescent protein
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although...
Fluorescent protein - Wikipedia
Laser capture microdissection
Laser capture microdissection (LCM), also called microdissection, laser microDissection (LMD), or laser-assisted microdissection (LMD or LAM) is a method for isolating specific cells of interest from ...
Laser capture microdissection - Wikipedia
Multifocal plane microscopy
Multifocal plane microscopy (MUM) or Multiplane microscopy or Biplane microscopy is a form of light microscopy that allows the tracking of the 3D dynamics in live cells at high temporal and spatial re...
Multifocal plane microscopy - Wikipedia
7-Aminoactinomycin D
7-Aminoactinomycin D (7-AAD) is a fluorescent chemical compound with a strong affinity for DNA. It is used as a fluorescent marker for DNA in fluorescence microscopy and flow cytometry. It intercala...
7-Aminoactinomycin D - Wikipedia
Two-photon excitation microscopy
Two-photon excitation microscopy is a fluorescence imaging technique that allows imaging of living tissue up to a very high depth, up to about one millimeter. Being a special variant of the multiphoto...
Two-photon excitation microscopy - Wikipedia
Phasor approach to fluorescence lifetime and spectral imaging
Phasor approach refers to a method which is used for vectorial representation of sinusoidal waves like alternative currents and voltages or electromagnetic waves. The amplitude and the phase of the w...
Phasor approach to fluorescence lifetime and spectral imaging - Wikipedia
Actinomycin
Dactinomycin (INN, BAN, AAN and USAN also known generically as actinomycin D), is the most significant member of actinomycines, which are a class of polypeptide antitumor antibiotics isolated from soi...
Actinomycin - Wikipedia
Quantitative phase contrast microscopy
Quantitative phase contrast microscopy is the collective name for a group of microscopy methods that quantify the phase shift that occurs when light waves pass through a more optically dense object.Tr...
Quantitative phase contrast microscopy - Wikipedia
Fluorescent microscopy
A fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to study properties of organic or inorganic subst...
Fluorescent microscopy - Wikipedia
Pulse-chase analysis
In biochemistry and molecular biology, a pulse-chase analysis is a method for examining a cellular process occurring over time by successively exposing the cells to a labeled compound (pulse) and then...
Pulse-chase analysis - Wikipedia
Photoactivated localization microscopy
Photo-activated localization microscopy (PALM or FPALM)and stochastic optical reconstruction microscopy (STORM) are widefield (as opposed to point scanning techniques such as laser scanning confocal m...
Photoactivated localization microscopy - Wikipedia
Confocal laser scanning microscopy
Confocal laser scanning microscopy (CLSM or LSCM) is a technique for obtaining high-resolution optical images with depth selectivity. The key feature of confocal microscopy is its ability to acquire i...
Fura-2-acetoxymethyl ester
Fura-2-acetoxymethyl ester, often abbreviated Fura-2AM, is a membrane-permeable derivative of the ratiometric calcium indicator Fura-2 used in biochemistry to measure cellular calcium concentrations b...
Fura-2-acetoxymethyl ester - Wikipedia
Time-lapse microscopy
Time-lapse microscopy is time-lapse photography applied to microscopy.Microscope image sequences are recorded and then viewed at a greater speed togive an accelerated view of the microscopic process.B...
Time-lapse microscopy - Wikipedia
GCaMP
GCaMP is a genetically encoded calcium indicator, or GECI initially developed by Junichi Nakai. GCaMP is created from a fusion of green fluorescent protein (GFP), calmodulin, and M13, a peptide seque...
GCaMP - Wikipedia