Fast-moving cell processes can be seen in 3D for the first time
The new lattice sheet microscope shoots high-res pictures of biological processes so quickly that fast-moving cells can be seen in 3D for the first time. The hardware is even able to produce video cli...
Exploring the universe of the cell; The 2012 GE Healthcare Cell Imaging Competition images
An animated film of the beautiful cell images that competed in the 2012 Cell Imaging Competition; first shown in Times Square New York, 20th April 2013. GE H...
'Gentle' microscope captures tiny life in action
Microscopists have been able to peer deep into cells, thanks to fluorescent molecules that stick to cellular structures. But the powerful light sources—often lasers—required to activate the fluorescen...
Cell imaging - Microscopic cell imaging jumps to 3D movies; unobtainium not required
Looking at cells just got a whole lot more interesting than just looking at a flat, super magnified image. Two researchers from the École Polytechnique Fédérale de Lausanne (EPFL) have f...
Staining
Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological t...
Staining - Wikipedia
Live cell imaging
Live cell imaging is the study of living cells using time-lapse microscopy. It is used by scientists to obtain a better understanding of biological function through the study of cellular dynamics. Liv...
Live cell imaging - Wikipedia
Phase contrast microscopy
Phase contrast microscopy is an optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image. Phase shifts themselves are ...
Phase contrast microscopy - Wikipedia
Digital holographic microscopy
Digital holographic microscopy (DHM) is digital holography applied to microscopy. Digital holographic microscopy distinguishes itself from other microscopy methods by not recording the projected image...
Digital holographic microscopy - Wikipedia
Fluorescence microscope
A fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to study properties of organic or inorganic subst...
Fluorescence microscope - Wikipedia
Light sheet fluorescence microscopy
Light sheet fluorescence microscopy (LSFM) is a fluorescence microscopy technique with an intermediate optical resolution, but good optical sectioning capabilities and high speed. In contrast to epifl...
Light sheet fluorescence microscopy - Wikipedia
Lattice light-sheet microscopy
Lattice light-sheet microscopy is a modified version of light sheet fluorescence microscopy that increases image acquisition speed while decreasing damage to cells caused by phototoxicity. This is ac...
Fluo-3
Fluo-3 is a fluorescence indicator of intracellular calcium (Ca). It is used to measure Ca inside living cells in flow cytometry and confocal laser scanning microscopy using visible light excitation (...
Fura-2
Fura-2, an aminopolycarboxylic acid, is a ratiometric fluorescent dye which binds to free intracellular calcium. It was the first widely used dye for calcium imaging, and remains very popular. Fura-2...
Fura-2 - Wikipedia
Actinomycin
Dactinomycin (INN, BAN, AAN and USAN also known generically as actinomycin D), is the most significant member of actinomycines, which are a class of polypeptide antitumor antibiotics isolated from soi...
Actinomycin - Wikipedia
Multiphoton fluorescence microscope
A multiphoton fluorescence microscope (MFM) is a specialized optical microscope.
The MFM uses pulsed long-wavelength light to excite fluorophores within the specimen being observed. The fluoropho...
Second-harmonic imaging microscopy
Second-harmonic imaging microscopy (SHIM) is based on a nonlinear optical effect known as second-harmonic generation (SHG). SHIM has been established as a viable microscope imaging contrast mechanism...
Laser capture microdissection
Laser capture microdissection (LCM), also called microdissection, laser microDissection (LMD), or laser-assisted microdissection (LMD or LAM) is a method for isolating specific cells of interest from ...
Laser capture microdissection - Wikipedia
7-Aminoactinomycin D
7-Aminoactinomycin D (7-AAD) is a fluorescent chemical compound with a strong affinity for DNA. It is used as a fluorescent marker for DNA in fluorescence microscopy and flow cytometry. It intercala...
7-Aminoactinomycin D - Wikipedia
Phasor approach to fluorescence lifetime and spectral imaging
Phasor approach refers to a method which is used for vectorial representation of sinusoidal waves like alternative currents and voltages or electromagnetic waves. The amplitude and the phase of the w...
Phasor approach to fluorescence lifetime and spectral imaging - Wikipedia
Nanovid microscopy
Nanovid microscopy, from “nanometer video-enhanced microscopy”, is a microscopic technique aimed at visualizing colloidal gold particles of 20-40 nm diameter (nanogold, immunogold) as dynamic mar...
Quantitative phase contrast microscopy
Quantitative phase contrast microscopy is the collective name for a group of microscopy methods that quantify the phase shift that occurs when light waves pass through a more optically dense object.Tr...
Quantitative phase contrast microscopy - Wikipedia
Fluorescent microscopy
A fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to study properties of organic or inorganic subst...
Fluorescent microscopy - Wikipedia
JUNQ and IPOD
JUNQ and IPOD are types of cytosolic protein inclusion bodies in eukaryotes.Neurodegenerative diseases, such as Parkinson’s, Alzheimer’s, and Huntington’s, are associated and correlated with protein a...
JUNQ and IPOD - Wikipedia
Pulse-chase analysis
In biochemistry and molecular biology, a pulse-chase analysis is a method for examining a cellular process occurring over time by successively exposing the cells to a labeled compound (pulse) and then...
Pulse-chase analysis - Wikipedia
Photoactivated localization microscopy
Photo-activated localization microscopy (PALM or FPALM)and stochastic optical reconstruction microscopy (STORM) are widefield (as opposed to point scanning techniques such as laser scanning confocal m...
Photoactivated localization microscopy - Wikipedia
Confocal laser scanning microscopy
Confocal laser scanning microscopy (CLSM or LSCM) is a technique for obtaining high-resolution optical images with depth selectivity. The key feature of confocal microscopy is its ability to acquire i...
Calcium imaging
Calcium imaging is a scientific technique usually carried out in research which is designed to show the calcium (Ca) status of a cell, tissue or medium. Calcium imaging techniques take advantage of s...
Brainbow
Brainbow is the process by which individual neurons in the brain can be distinguished from neighboring neurons using fluorescent proteins. By randomly expressing different ratios of red, green, and bl...
Brainbow - Wikipedia
Green fluorescent protein
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although...
Green fluorescent protein - Wikipedia
Synapto-pHluorin
Synapto-pHluorin is a genetically encoded optical indicator of vesicle release and recycling. It is used in neuroscience to study transmitter release. It consists of a pH-sensitive form of green fluor...